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Literature summary extracted from
- Whole-genome sequencing, genome mining, metabolic reconstruction and evolution of pentachlorophenol and other xenobiotic degradation pathways in Bacillus tropicus strain AOA-CPS1 (2021), Funct. Integr. Genomics, 21, 171-193 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.13.50 | analogues of PCP catabolic genes (cpsABCDE, and p450) are successfully amplified from the whole genome of BtAOA using the primers designed based on the gene sequences retrieved from the whole genome annotation data submitted at NCBI | Bacillus tropicus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.50 | 0.17 | - |
Pentachlorophenol | recombinant CpsB, pH 7.0, 30°C | Bacillus tropicus |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.50 | pentachlorophenol + NADH + H+ + O2 | Bacillus tropicus | - |
2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O | - |
? | |
| 1.14.13.50 | pentachlorophenol + NADH + H+ + O2 | Bacillus tropicus AOA-CPS1 | - |
2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.50 | Bacillus tropicus | - |
- |
- |
| 1.14.13.50 | Bacillus tropicus AOA-CPS1 | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.50 | pentachlorophenol + NADH + H+ + O2 | - |
Bacillus tropicus | 2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O | - |
? | |
| 1.14.13.50 | pentachlorophenol + NADH + H+ + O2 | - |
Bacillus tropicus AOA-CPS1 | 2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.13.50 | ? | x * 64200, about, SDS-PAGE | Bacillus tropicus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.13.50 | CpsB | - |
Bacillus tropicus |
| 1.14.13.50 | GM610_18120 | locus name | Bacillus tropicus |
| 1.14.13.50 | PCP 4-monooxygenase | - |
Bacillus tropicus |
| 1.14.13.50 | PCP-4-monooxygenase | - |
Bacillus tropicus |
| 1.14.13.50 | PcpB | - |
Bacillus tropicus |
| 1.14.13.50 | Phe4MO | - |
Bacillus tropicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.50 | 30 | - |
- |
Bacillus tropicus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.50 | 25 | 30 | stable at | Bacillus tropicus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.50 | 4.58 | - |
Pentachlorophenol | recombinant CpsB, pH 7.0, 30°C | Bacillus tropicus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.50 | 7 | - |
- |
Bacillus tropicus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.50 | 6 | 7.5 | stable at | Bacillus tropicus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.50 | NADH | - |
Bacillus tropicus | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.14.13.50 | Bacillus tropicus | sequence calculation | - |
5.84 |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.13.50 | evolution | evolution of PCP catabolic genes in BtAOA, overview | Bacillus tropicus |
| 1.14.13.50 | metabolism | two pathways are proposed for pentachlorophenol (PCP) transformation in BtAOA. In the first pathway, 2,6-bis(1,1-dimethylethyl)phenol, 1-methoxy-5-trimetylsilyoxyhexane, trimethylsilyl 2-butoxyacetate and methyl 2-hydroxyl-3-methylbutanoate fragments are found. The metabolites detected for the proposed second pathway include 1,3-dimethyl-4,6-diisopropylbenzene, 2,4-dimethylbenzenecarboxaldehyde and 2,5-dimethylbenzaldehyde. Both 2,6-di-tert-butylbenzoquinone and 1,3-dimethyl-4,6-diisopropylbenzene cannot be metabolites from the same pathway, it could be that, one of the pathways is initiated by PcpB and the other by cytochrome P450 monooxygenase. Metabolic reconstruction of BtAOA xenobiotics biodegradation pathways, pathway regulation analysis, overview | Bacillus tropicus |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.50 | 26.94 | - |
Pentachlorophenol | recombinant CpsB, pH 7.0, 30°C | Bacillus tropicus | |
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